A head-mountable microscope for high-speed fluorescence brain imaging

In this paper we present a rodent head-mountable, fully self contained imaging device that is capable of recording high speed fluorescent signals from genetically-encoded probes or voltage sensitive dyes. This device enables the study of neuronal activity in awake and mobile animals during more natural behaviors without the stress and suppression of anesthesia and restraints. The device is a fully self contained fluorescence microscope, illumination system and image sensor. The total weight of the system is 10 g and is capable of imaging up to 890 fps. We are currently running voltage dye experiments using the system on the somatosensory cortex of a mouse during whisker stimulation.