An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers

Author

Krishnan, R. ; Charlot, D. ; Kumosa, L. ; Hanna, W. ; Lu, J. ; Sonnenberg, A. ; Heller, M.

Author_Institution

Biol. Dynamics, Inc., San Diego, CA, USA

fYear

2011

fDate

10-12 Nov. 2011

Firstpage

373

Lastpage

376

Abstract

The ability to rapidly detect cell free circulating (cfc) DNA and other nanoparticulate disease biomarkers directly in blood is a major challenge for nanomedicine. We now show that AC Electrokinetic (ACE) microelectrode array devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates directly from whole blood and other high conductance samples (plasma, serum etc.). The device shows isolation of naked DNA in water and plasma at >;500bp size and can isolate DNA down to 50 picograms in 50uL of fluid. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia.

Keywords

DNA; biomedical electrodes; blood; cancer; diseases; electrokinetic effects; microelectrodes; molecular biophysics; nanomedicine; AC electrokinetic microelectrode array devices; Chronic Lymphocytic Leukemia; blood; cancer related DNA nanoparticulate biomarker detection; cell free circulating DNA; nanomedicine; nanoparticulate disease biomarkers; plasma; serum; water; Biomarkers; Blood; DNA; Fluorescence; Microelectrodes; Plasmas;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Circuits and Systems Conference (BioCAS), 2011 IEEE

Conference_Location

San Diego, CA

Print_ISBN

978-1-4577-1469-6

Type

conf

DOI

10.1109/BioCAS.2011.6107805

Filename

6107805