Title :
An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers
Author :
Krishnan, R. ; Charlot, D. ; Kumosa, L. ; Hanna, W. ; Lu, J. ; Sonnenberg, A. ; Heller, M.
Author_Institution :
Biol. Dynamics, Inc., San Diego, CA, USA
Abstract :
The ability to rapidly detect cell free circulating (cfc) DNA and other nanoparticulate disease biomarkers directly in blood is a major challenge for nanomedicine. We now show that AC Electrokinetic (ACE) microelectrode array devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates directly from whole blood and other high conductance samples (plasma, serum etc.). The device shows isolation of naked DNA in water and plasma at >;500bp size and can isolate DNA down to 50 picograms in 50uL of fluid. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia.
Keywords :
DNA; biomedical electrodes; blood; cancer; diseases; electrokinetic effects; microelectrodes; molecular biophysics; nanomedicine; AC electrokinetic microelectrode array devices; Chronic Lymphocytic Leukemia; blood; cancer related DNA nanoparticulate biomarker detection; cell free circulating DNA; nanomedicine; nanoparticulate disease biomarkers; plasma; serum; water; Biomarkers; Blood; DNA; Fluorescence; Microelectrodes; Plasmas;
Conference_Titel :
Biomedical Circuits and Systems Conference (BioCAS), 2011 IEEE
Conference_Location :
San Diego, CA
Print_ISBN :
978-1-4577-1469-6
DOI :
10.1109/BioCAS.2011.6107805
