DocumentCode :
2717603
Title :
Measuring biophysical properties of living cells with digital holographic microscopy
Author :
Marquet, Pierre ; Kühn, Jonas ; Boss, Daniel ; Jourdain, Pascal ; Magistretti, Pierre ; Depeursinge, Christian
Author_Institution :
Centre de Neurosciences Psychiatriques, Univ. of Lausanne, Prilly-Lausanne, Switzerland
fYear :
2011
fDate :
19-24 June 2011
Firstpage :
1
Lastpage :
4
Abstract :
Quantitative Phase Imaging techniques including digital holographic microscopy (DHM) have emerged recently in life sciences and can be aimed at monitoring and quantifying, in a non-invasive way, cellular processes. Due to its interferometric nature, the DHM phase signal, which depends on both cell thickness and integral RI can be measured with a high sensitivity. However, this dual origin makes its interpretation in terms of the underlying cell process difficult. Consequently, we propose a dual wavelength digital holographic microscopy setup to measure in a single shot fashion cellular thickness and integral RI of living cells. The method is based on the use of an absorbing dye inducing a high RI dispersion in the extracellular medium at the two recording wavelengths. Consequently, from the two quantitative phase signals, measured for the two wavelengths, Posters cellular RI and thickness can be unambiguously calculated.
Keywords :
bio-optics; biological techniques; cellular biophysics; holographic interferometry; optical microscopy; RI dispersion; absorbing dye; biophysical properties; cell process; cellular processes; cellular thickness; digital holographic microscopy; dual wavelength digital holographic microscopy; life sciences; living cells; noninvasive way; quantitative phase imaging techniques; Dispersion; Extracellular; Image reconstruction; Microscopy; Optimized production technology; Phase measurement; Refractive index;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Information Optics (WIO), 2011 10th Euro-American Workshop on
Conference_Location :
Benicassim
Print_ISBN :
978-1-4577-1227-2
Electronic_ISBN :
978-1-4577-1225-8
Type :
conf
DOI :
10.1109/WIO.2011.5981450
Filename :
5981450
Link To Document :
بازگشت