Zebrafish Cell/Neuron Quantitation by Modified Circular Hough Transform

Author

Liu, Tianming ; Lu, Jianfeng ; Nie, Jingxin ; Li, Gang ; Yan, Hui ; Yang, Jingyu ; Guo, Lei ; Xia, Weiming ; Wong, Stephen T C

Author_Institution

Center for Bioinformatics, Harvard Med. Sch., Boston, MA

fYear

2006

fDate

38899

Firstpage

1

Lastpage

2

Abstract

Microscopic imaging has become a critical step in the analysis of zebrafish phenotype, but tremendous amount of images generated from large number of embryos have become a bottleneck of data interpretation. The availability of an image informatics pipeline will be a significant step towards achieving reproducible quantitation of zebrafish phenotypes in scientific research and high-throughput screening. This paper presents an automated algorithm based on modified circular Hough transform for quantitation of zebrafish cells and neurons in microscopy imaging. Experimental results of three datasets are shown to demonstrate the performance of the algorithm. This algorithm has been integrated into our CBI Platform

Keywords

Hough transforms; cellular biophysics; medical image processing; neurophysiology; cell quantitation; image informatics; microscopic imaging; modified circular Hough transform; neuron quantitation; zebrafish; Bioinformatics; Biomedical imaging; Embryo; Image edge detection; Microscopy; Molecular imaging; Neurons; Pixel; Radiology; Transforms;

fLanguage

English

Publisher

ieee

Conference_Titel

Life Science Systems and Applications Workshop, 2006. IEEE/NLM

Conference_Location

Bethesda, MD

Print_ISBN

1-4244-0277-8

Electronic_ISBN

1-4244-0278-6

Type

conf

DOI

10.1109/LSSA.2006.250413

Filename

4015814