In situ Biomolecule Assembly and Activity within Completely Packaged Microfluidic Devices

Author

Park, Jung Jin ; Luo, Xiaolong ; Yi, Hyunmin ; Ghodssi, Reza ; Rubloff, Gary W.

Author_Institution

Dept. of Mater. Sci. & Eng., Maryland Univ., College Park, MD

fYear

2006

fDate

38899

Firstpage

1

Lastpage

2

Abstract

We demonstrate in situ biomolecule assembly in a completely packaged microfluidic bioMEMS (micro electro mechanical systems) device and the resulting biofunctionality of the system. Chitosan biopolymer is employed as the platform for biomolecules, using its pH responsive properties for site-selective electrodeposition and it amine functionality for biomolecular conjugation. These characteristics, combined with a robust device and packaging technology, provide the basis to emulate multi-step, multi-site bioreactions as needed for metabolic engineering. We demonstrate the directed assembly and functionality of biomolecules in bioMEMS through fluorescence of species and enzymatic conversion of small molecules

Keywords

bioMEMS; biochemistry; biological techniques; enzymes; fluorescence; microfluidics; molecular biophysics; pH; polymers; amine functionality; bioMEMS; biofunctionality; biomolecular conjugation; chitosan biopolymer; completely packaged microfluidic devices; enzymatic conversion; fluorescence; in situ biomolecule assembly; metabolic engineering; microelectromechanical systems; multi-step multi-site bioreactions; pH responsive properties; site-selective electrodeposition; Assembly systems; Biochemistry; Electrodes; Fluorescence; Microchannel; Microfluidics; Molecular biophysics; Optical surface waves; Packaging; Robustness;

fLanguage

English

Publisher

ieee

Conference_Titel

Life Science Systems and Applications Workshop, 2006. IEEE/NLM

Conference_Location

Bethesda, MD

Print_ISBN

1-4244-0277-8

Electronic_ISBN

1-4244-0278-6

Type

conf

DOI

10.1109/LSSA.2006.250370

Filename

4015841