Title :
In situ Biomolecule Assembly and Activity within Completely Packaged Microfluidic Devices
Author :
Park, Jung Jin ; Luo, Xiaolong ; Yi, Hyunmin ; Ghodssi, Reza ; Rubloff, Gary W.
Author_Institution :
Dept. of Mater. Sci. & Eng., Maryland Univ., College Park, MD
Abstract :
We demonstrate in situ biomolecule assembly in a completely packaged microfluidic bioMEMS (micro electro mechanical systems) device and the resulting biofunctionality of the system. Chitosan biopolymer is employed as the platform for biomolecules, using its pH responsive properties for site-selective electrodeposition and it amine functionality for biomolecular conjugation. These characteristics, combined with a robust device and packaging technology, provide the basis to emulate multi-step, multi-site bioreactions as needed for metabolic engineering. We demonstrate the directed assembly and functionality of biomolecules in bioMEMS through fluorescence of species and enzymatic conversion of small molecules
Keywords :
bioMEMS; biochemistry; biological techniques; enzymes; fluorescence; microfluidics; molecular biophysics; pH; polymers; amine functionality; bioMEMS; biofunctionality; biomolecular conjugation; chitosan biopolymer; completely packaged microfluidic devices; enzymatic conversion; fluorescence; in situ biomolecule assembly; metabolic engineering; microelectromechanical systems; multi-step multi-site bioreactions; pH responsive properties; site-selective electrodeposition; Assembly systems; Biochemistry; Electrodes; Fluorescence; Microchannel; Microfluidics; Molecular biophysics; Optical surface waves; Packaging; Robustness;
Conference_Titel :
Life Science Systems and Applications Workshop, 2006. IEEE/NLM
Conference_Location :
Bethesda, MD
Print_ISBN :
1-4244-0277-8
Electronic_ISBN :
1-4244-0278-6
DOI :
10.1109/LSSA.2006.250370
