Title :
A detection-based framework for the analysis of recycling in TIRF microscopy
Author :
Chessel, A. ; Cinquin, B. ; Bardin, S. ; Boulanger, J. ; Salamero, J. ; Kervrann, C.
Author_Institution :
Cell & Tissue Imaging Facility, Inst. Curie, Paris, France
Abstract :
Endocytosis/recycling and exocytosis are mechanisms conserved through evolution allowing cells to communicate with their external medium. In order to study these dynamic processes, the present work proposes a patch-based method for detecting recycling or exocytotic events at the Plasma membrane in fast TIRF microscopy combined with the computation of normalized temporal representations of those events. Evaluation, performed on TIRF sequences showing Transferrin receptor (TfR) recycling, validates a high detection rate fully compatible with an automatic data extraction and analysis of the plasma membrane recycling process.
Keywords :
bio-optics; biomedical optical imaging; biomembranes; cellular biophysics; fluorescence; image sequences; medical image processing; molecular biophysics; optical microscopy; proteins; TIRF microscopy; TIRFM sequences; automatic data extraction; cells; endocytosis; exocytosis; fluorescent trans-membrane proteins; plasma membrane; recycling; total internal reflection fluorescence microscopy; transferrin receptor; Biological system modeling; Biomembranes; Event detection; Fluorescence; Iron; Microscopy; Plasma properties; Plasma waves; Protein engineering; Recycling; TIRFM; TfR; endocytosis/recycling; event detection; patch-based;
Conference_Titel :
Biomedical Imaging: From Nano to Macro, 2010 IEEE International Symposium on
Conference_Location :
Rotterdam
Print_ISBN :
978-1-4244-4125-9
Electronic_ISBN :
1945-7928
DOI :
10.1109/ISBI.2010.5490230
