DocumentCode :
2721985
Title :
Muscle histology image analysis for sarcopenia: Registration of successive sections with distinct atpase activity
Author :
Sertel, O. ; Dogdas, B. ; Chi Sung Chiu ; Gurcan, M.N.
fYear :
2010
fDate :
14-17 April 2010
Firstpage :
1423
Lastpage :
1426
Abstract :
One way of evaluating muscle quality is to determine its fiber type composition in histological sections. A complete muscle fiber type characterization system requires combining information from successive muscle histology images with different ATPase stain. Due to the local and global deformations introduced in slide preparation process, a precise non-rigid registration is essential to construct the spatial correspondences between these successive images. This study proposes an approach for automated non-rigid registration of successive muscle histological sections. We propose a feature-based registration that uses a two stage approach: a rigid initialization followed by a non-rigid refinement. The rigid initialization step globally aligns successive tissue slides by finding correspondences between individually segmented muscle fibers using Fourier shape descriptors and computing the global rigid transformation using a voting scheme tolerant to mismatches. In the non-rigid stage we establish precise point correspondences using the normalized cross correlation metric and compute the non-rigid distortion using a polynomial transformation that minimizes the mean square distance between these control points.
Keywords :
Fourier transforms; feature extraction; image registration; medical image processing; muscle; ATPase activity; Fourier shape descriptors; global rigid transformation; image analysis; muscle fibers; muscle histology; nonrigid distortion; normalized cross correlation; polynomial transformation; precise nonrigid registration; sarcopenia; Biology computing; Image analysis; Image registration; Laboratories; Microscopy; Muscles; Mutual information; Polynomials; Shape; Voting; histological image registration; muscle fiber typing; sarcopenia;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Biomedical Imaging: From Nano to Macro, 2010 IEEE International Symposium on
Conference_Location :
Rotterdam
ISSN :
1945-7928
Print_ISBN :
978-1-4244-4125-9
Type :
conf
DOI :
10.1109/ISBI.2010.5490266
Filename :
5490266
Link To Document :
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