Study of Response to Zinc in Human Prostate Spheroids Using X-Ray Microfluorescence

Author

Leitao, Roberta ; Santos, Carlos Antonio ; Palumbo, AntoÌ‚nio ; Souza, Pedro ; Leitao Canellas, Catarine G. ; Anjos, Marcelino J. ; Nasciutti, Luiz E. ; Lopes, Ricardo T.

Author_Institution

Nucl. Instrum. Lab., Fed. Univ. of Rio de Janeiro, Rio de Janeiro, Brazil

Volume

64

Issue

8

fYear

2015

fDate

Aug. 2015

Firstpage

2121

Lastpage

2126

Abstract

Diseases of the prostate gland are the most frequently diagnosed in men after middle age. Spheroid cell culture is a useful technique for tissue engineering or regenerative medicine research, pharmacological and toxicological studies, and fundamental studies in cell biology. In this paper, we investigated zinc distribution in cell spheroids in benign prostate hyperplasia (BPH) and prostate cancer (PCa) (DU145) and analyzed the differences in the response to zinc (0-150 μM) treatment using μXRF with synchrotron radiation. The results showed nonuniform distribution of Zn in all the spheroids analyzed. The differential response to zinc of BPH and DU145 cell spheroids suggests that zinc may have an important role in PCa.

Keywords

X-ray fluorescence analysis; biochemistry; cancer; cellular biophysics; patient diagnosis; zinc; μXRF; BPH; DU145 cell spheroids; X-ray microfluorescence; Zn; benign prostate hyperplasia; diseases; human prostate spheroids; prostate cancer; spheroid cell culture; synchrotron radiation; zinc distribution; Diseases; Principal component analysis; Prostate cancer; Synchrotron radiation; Zinc; Cell spheroids; X-ray fluorescence; X-ray fluorescence.; elemental distribution; prostate; synchrotron radiation;

fLanguage

English

Journal_Title

Instrumentation and Measurement, IEEE Transactions on

Publisher

ieee

ISSN

0018-9456

Type

jour

DOI

10.1109/TIM.2015.2408797

Filename

7115100