A microfluidic channel for cell separation

The work presented in this paper is an extension of earlier work, which described the utility of dielectrophoresis (DEP) to affect cell fractionation in a relatively large fluidic channel. The focus of our current work, described in this paper, is to utilize glass microchip prototyping technology for microfluidics, provided by Micralyne, Canada (ProtoChip), to fabricate microfluidic channel to facilitate DEP fractionation. More specifically, we examine in detail the design and the process involved in the fabrication of the microfluidic components, such as the separation channel in the glass chip. In addition, we examine the testing of microfluidic components and describe their performance and capabilities to affect cell separation.