Title :
Protein Conformational Dynamics Measured With Terahertz Time Domain Spectroscopy
Author :
Knab, Joseph R. ; Chen, Jing-Yin ; Ye, Shuji ; He, Yunfen ; Markelz, Andrea G.
Author_Institution :
Univ. at Buffalo, Buffalo
Abstract :
Tertiary structural vibrational modes of proteins are at far infrared or terahertz frequencies. These modes involve collective motion of many atoms and are indicative of the protein structure, hydration and binding with ligands and other proteins. The measurements of conformational change have focused on the hydration, denaturing effects, and photoactive proteins bacteriorhodopsin. In addition, a strong sensitivity of the terahertz response to heme protein oxidation state is shown. The impact of the results on biomolecular switching mechanisms as well as applications to biomolecular sensing is discussed.
Keywords :
biochemistry; biological techniques; molecular biophysics; molecular configurations; oxidation; proteins; submillimetre wave spectroscopy; biomolecular sensing; biomolecular switching mechanisms; denaturing effects; heme protein oxidation state; photoactive proteins bacteriorhodopsin; protein binding; protein conformational dynamics; protein hydration; protein structure; terahertz time domain spectroscopy; tertiary structural vibrational modes; vibrational mode density; Biomedical equipment; Dielectrics; Frequency measurement; Helium; Medical services; Proteins; Spectroscopy; Submillimeter wave measurements; Time measurement; Vibration measurement;
Conference_Titel :
Infrared Millimeter Waves and 14th International Conference on Teraherz Electronics, 2006. IRMMW-THz 2006. Joint 31st International Conference on
Conference_Location :
Shanghai
Print_ISBN :
1-4244-0400-2
Electronic_ISBN :
1-4244-0400-2
DOI :
10.1109/ICIMW.2006.368391
