Poster: Empirical comparison of protocols for sequencing-based gene and isoform expression profiling

In this article, isoform expression inference, RNA-Seq and DGE gave comparable results whereas for gene expression DGE has better accuracy when enough isoforms are cut by the restriction enzyme used. DGE accuracy improves with the percentage of isoforms cut and is better for partial digestion compared to complete digestion. Using enzymes with degenerate recognition sites, such as CviJI, yields better accuracy than using enzymes from published studies.