Title :
Directed Evolution of AAV Mutants for Enhanced Gene Delivery
Author :
Schaffer, D.V. ; Maheshri, N. ; Schaffer, David V. ; Maheshri, N.
Author_Institution :
Department of Chemical Engineering and the Helen Wills Neuroscience Institute, University of California at Berkeley, Berkeley, CA, USA
Abstract :
Gene therapy vehicles must be engineered to overcome numerous barriers that limit delivery efficiency. These barriers arise at every step of the delivery process, including the transit of the vector from injection to a cell surface, re ceptor binding and uptake, intracellular trafficking, and nuclear entry. The gene transfer properties of the highly promising adeno-associated viral (AAV) vector at each step are determined by its capsid structure.
Keywords :
AAV; directed evolution; gene delivery; viral vector; Assembly; Bioinformatics; DNA; Genomics; Humans; Libraries; Medical treatment; Packaging; Proteins; Rabbits;
Conference_Titel :
Engineering in Medicine and Biology Society, 2004. IEMBS '04. 26th Annual International Conference of the IEEE
Print_ISBN :
0-7803-8439-3
DOI :
10.1109/IEMBS.2004.1403990
