Model-based 3-D Reconstructions for the Structural Biology of Viruses Exhibiting Incompatible Symmetries

Author

Prust, Cory J. ; Doerschuk, Peter C. ; Johnson, John E.

Author_Institution

Sch. of Electr. & Comput. Eng., Purdue Univ., West Lafayette, IN

fYear

2006

fDate

7-10 May 2006

Firstpage

404

Lastpage

408

Abstract

A model-based approach to 3-D reconstruction from cryo electron microscope images is presented for the case of a virus particle lacking total particle symmetry. In particular, we consider the reconstruction of a tailed bacteriophage where the symmetries of the capsid and tail are incompatible. We describe a model-based statistical approach and a two-stage reconstruction algorithm. Numerical results from synthetic data are presented

Keywords

biological techniques; biology computing; electron microscopy; image reconstruction; microorganisms; statistical analysis; 3D reconstructions; capsid; cryo electron microscope images; image reconstruction; statistical approach; structural biology; tail; tailed bacteriophage; total particle symmetry; two-stage reconstruction algorithm; viruses; Assembly; Biological system modeling; Computational biology; Electrons; Fourier transforms; Image reconstruction; Particle scattering; Tail; Three dimensional displays; Viruses (medical);

fLanguage

English

Publisher

ieee

Conference_Titel

Electro/information Technology, 2006 IEEE International Conference on

Conference_Location

East Lansing, MI

Print_ISBN

0-7803-9592-1

Electronic_ISBN

0-7803-9593-X

Type

conf

DOI

10.1109/EIT.2006.252192

Filename

4017729