Fulvic acid fractions from mumie

Fulvic acids (FA) from mumie (shilajit) were fractionated by size exclusion chromatography and characterized by IR, NMR ¹H and mass spectra. The presence of functional groups that are usually responsible for antiradical groups that are usually responsible for antiradical action was indicated. Special investigation of antiradical activity of the fractions has been undertaken. It was performed spectrophotometrically by superoxide radical generation with xanthine/xantine oxidase enzyme system using nitroblue tetrazolium chloride as a spectroscopic indicator. First order kinetics of superoxide radical formation allowed to easily determine the inhibition of the reaction by FA fractions from mumie. The antiradical activity is discussed.