DocumentCode :
2748286
Title :
Multiple volume injection technique for high-resolution DNA sample detection utilizing planar microfluidic chip
Author :
Fu, Lung-Ming ; Lin, Che-Hsin ; Chang, Guan-Liang
Author_Institution :
Nat. Pingtung Univ. of Sci. & Technol., Taiwan
Volume :
2
fYear :
2004
fDate :
1-5 Sept. 2004
Firstpage :
5017
Lastpage :
5020
Abstract :
This paper presents an experimental and numerical investigation into the use of high-resolution injection techniques to separate DNA fragments within electrophoresis microchips. The study addresses the principal material transport mechanisms such as electrokinetic migration, fluid flow, diffusion, variable-volume injection methods, and gives detail analyses to the double-L injection technique, which employs electrokinetic manipulations to avoid sample leakage within the microchip. We also study the sample leakage effect during sample injection and separation. The standard DNA ladder and the φ-174 DNA fragments are used to test the performance for this proposed method. Results show that this unique injection system in the current microfluidic chip presented within this paper is capable of simulating the functions of the cross, double-T form through appropriate manipulations of the electric field within its various channels. The proposed double-L injection method confirms its ability to reduce sample leakage effect during operation. The integrated microfluidic chip and double-L injection technique developed in this study has an exciting potential for use in high-quality, high-throughput chemical analysis applications and in many other applications throughout the field of micro-total-analysis systems.
Keywords :
DNA; biodiffusion; biological techniques; cellular transport; electrophoresis; microfluidics; diffusion; double-L injection technique; electrokinetic migration; electrophoresis microchips; fluid flow; high-resolution DNA sample detection; material transport mechanisms; multiple volume injection technique; planar microfluidic chip; variable-volume injection methods; Biomedical engineering; DNA; Electrokinetics; Fluorescence; Microchannel; Microfluidics; Microscopy; Plugs; Solvents; Voltage; capillary electrophoresis; electroosmotic flow; electrophoresis; micro-total-analysis systems; microchip;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Engineering in Medicine and Biology Society, 2004. IEMBS '04. 26th Annual International Conference of the IEEE
Conference_Location :
San Francisco, CA
Print_ISBN :
0-7803-8439-3
Type :
conf
DOI :
10.1109/IEMBS.2004.1404386
Filename :
1404386
Link To Document :
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