Title :
Design and characterization of polyaniline:Poly(L-lactic) acid thin films for stem cell monitoring applications
Author :
Pujol, M. Pilar ; Rindone, Alexandra N. ; Cooper, James A. ; Lewis, Kim M.
Author_Institution :
Dept. of Phys., Appl. Phys., & Astron., Rensselaer Polytech. Inst., Troy, NY, USA
Abstract :
Human mesenchymal stem (hMSC) cells have demonstrated a use for regenerative and therapeutic treatments; yet, more effective methods are needed to homogenize differentiated hMSCs to ensure their reliability in a clinical setting. This study focused on developing an electroconductive platform that would enable precise monitoring of hMSCs differentiation via conductive atomic force microscopy. Polyaniline:poly(L-lactic) acid (PANi:PLLA) thin films were fabricated and characterized to assess their potential to serve as an electroconductive substrate for hMSCs. Results from currentvoltage (I-V) measurements showed that films with PANi:PLLA mass ratios of 1:2 and 3:2 exhibited linear I-V behavior, which is characteristic of conductive materials. These electroconductive films will be used as an electroconductive platform to measure I-V curves of individual hMSCs and identify their state of differentiation.
Keywords :
atomic force microscopy; biomedical materials; cellular biophysics; polymer films; thin films; conductive atomic force microscopy; current-voltage curve measurements; electroconductive films; electroconductive substrate; human mesenchymal stem cell differentiation; human mesenchymal stem cell monitoring applications; polyaniline-poly(L-lactic) acid thin film characterization; polyaniline-poly(L-lactic) acid thin film fabrication; regenerative treatments; therapeutic treatments; Conductivity; Current measurement; Electric potential; Films; Scanning electron microscopy; Stem cells; Substrates;
Conference_Titel :
Biomedical Engineering Conference (NEBEC), 2015 41st Annual Northeast
Conference_Location :
Troy, NY
Print_ISBN :
978-1-4799-8358-2
DOI :
10.1109/NEBEC.2015.7117190
