Automatic High Dynamic Range and Resolution Imaging for 2DGE Images

2-dimensional gel electrophoresis image (2DGE) has been commonly used by biologists. Biologists only take one image for each 2D gel in traditional, and separate complex protein mixtures in the image. The image took by traditional way shall loss some of proteins that are existing in the 2D gel originally. To reduce the loss, we propose a high dynamic range and resolution (HDRR) image technique in this paper. HDRR will preserve whole protein spots by taking multiple luminance and resolution images. We can change resolutions and luminance of HDRR image immediately. In traditionally, the generation of a HDRR image needs to scan a 2D gel many times in different resolutions and luminance. However, this way will be time-consuming in scanning and probably causes manual carelessness. The other contribution of the paper is our HDRR method can scan different resolutions and luminance as well as integrating these images in HDRR format automatically. The automatic method mentioned in this paper saves 2/3 time of traditional way in scanning. This is the other contribution of this paper