Title :
Flexible microfluidic-device-stamp-system with integrated electrical sensor for real time DNA detection
Author :
Dobrinski, H. ; Wilkens, M. ; Benecke, W. ; Binder, J.
Author_Institution :
Inst. fur Mikrosensoren, -aktuatoren und -systeme, Bremen Univ., Germany
Abstract :
Describes the design of a reusable microfluidic flow cell for real time DNA detection. This Microfluidic-Device-Stamp-System (MDSS) is realized by a microstructured Silicon-Polymer-Hybrid that is pressed onto a sensing element consists of thin metallic interdigitated electrodes with immobilized capture DNA for hybridization detection. The hybridization temperature is controlled by integrated heating and sensing elements manufactured with standard microsystem technologies. Two-dimensional FEM flow analyses using ANSYS 5.5/FLOTRAN were done to optimize the homogeneities of fluid flow and DNA mass transport in the sensing region
Keywords :
DNA; biological techniques; electric sensing devices; microfluidics; ANSYS 5.5/FLOTRAN; DNA mass transport; flexible microfluidic-device-stamp-system; fluid flow homogeneities optimization; hybridization detection; immobilized capture DNA; integrated electrical sensor; integrated heating/sensing elements; microstructured silicon-polymer-hybrid; real time DNA detection; reusable microfluidic flow cell; standard microsystem technologies; thin metallic interdigitated electrodes; two-dimensional FEM flow analyses; DNA; Electrodes; Heating; Microfluidics; Polymer films; Sensor arrays; Silicon; Substrates; Temperature control; Temperature sensors;
Conference_Titel :
Microtechnologies in Medicine and Biology, 1st Annual International, Conference On. 2000
Conference_Location :
Lyon
Print_ISBN :
0-7803-6603-4
DOI :
10.1109/MMB.2000.893735
