A stretchable Micro-Electrode Array for in vitro electrophysiology

A novel stretchable Micro-Electrode Array (MEA) for biomedical applications with robust spiraled interconnects and radial micro-grooves for cell alignment is presented, enabling the unique combination of alignment, mechanical stimulation, and electrical characterization of living cells. Many of the problems normally associated with the fabrication of interconnects and electrodes on stretchable PDMS (Polydimethylsiloxane) membranes have been eliminated by first fabricating the interconnects and then spin-coating the PDMS. Experiments supported by Finite Element simulations demonstrate the robustness of the new spiraled interconnect design. The alignment of cells to micro-molded grooves is demonstrated, allowing for directional stretching of the cells.