Title :
Label-free adhesion-based cell sorter using optimized oblique grooves for early cancer detection
Author :
Chen, N.H. ; Tomita, U. ; Kasagi, N. ; Nagamune, T. ; Suzuki, Y.
Author_Institution :
Dept. of Mech. Eng., Univ. of Tokyo, Tokyo, Japan
Abstract :
We report development of a label-free adhesion-based cell sorter for early cancer detection. The sorting method is based on specific adhesion between target cells and antibody immobilized on oblique grooves of the channel wall. By optimizing the groove dimensions, lateral cell displacement twice as large as our earlier report has been obtained. Continuous lateral separation of HeLa cancer cells from mouse whole blood has been demonstrated.
Keywords :
adhesion; bioMEMS; biological techniques; biomechanics; blood; cancer; cellular biophysics; microfabrication; optimisation; HeLa cancer cells; adhesion; antibody immobilization; blood; cancer detection; cell displacement; channel wall; continuous lateral separation; groove dimensions; label-free adhesion-based cell sorter; mouse; optimized oblique grooves; sorting method; target cells; Adhesives; Blood; Cancer; Cancer detection; Fluorescence; Mice; Sorting;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2011 IEEE 24th International Conference on
Conference_Location :
Cancun
Print_ISBN :
978-1-4244-9632-7
Electronic_ISBN :
1084-6999
DOI :
10.1109/MEMSYS.2011.5734572
