Low cost micro-platform for culturing and stimulation of cardiomyocyte tissue

Author

Oropeza-Ramos, L. ; Macìas, A. ; Juárez, S. ; Falcòn, A. ; Torres, A. ; Hautefeuille, M. ; González, H.

Author_Institution

Univ. Nac. Autonoma de Mexico (UNAM), Mexico City, Mexico

fYear

2011

fDate

23-27 Jan. 2011

Firstpage

912

Lastpage

915

Abstract

This paper reports a low cost rapid prototyping platform to culture cardiomyocyte tissue with embedded biocompatible stimulation electrodes based on micro-structured conductive PDMS. The polymeric platform was fabricated using conventional soft lithography based on a non-photolithographic method to obtain molds, combined with the inclusion of micro-structured polymeric electrodes in the replicas. Device fabrication, electrical characterizations of the conductive polymer, as well as mechanical response of cultured tissue under spontaneous and stimulated activity are presented. Through this method, synchronization and coupling bio-electromechanical activity of different cell aggregates has been observed.

Keywords

bioelectric phenomena; biological specimen preparation; biomechanics; biomedical electrodes; biomedical materials; conducting polymers; soft lithography; tissue engineering; biocompatible stimulation electrode; cardiomyocyte tissue culturing; cardiomyocyte tissue stimulation; conductive PDMS; device fabrication; electrical characterizations; microplatform; rapid prototyping platform; soft lithography; Aggregates; Biomedical optical imaging; Cardiology; Electrical stimulation; Electrodes; Polymers; Printers;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2011 IEEE 24th International Conference on

Conference_Location

Cancun

ISSN

1084-6999

Print_ISBN

978-1-4244-9632-7

Type

conf

DOI

10.1109/MEMSYS.2011.5734574

Filename

5734574