Towards a microfluidic dispenser chip for printing of single cells

Author

Yusof, A. ; Zengerle, R. ; Koltay, P.

Author_Institution

IMTEK, Univ. of Freiburg, Freiburg, Germany

fYear

2011

fDate

23-27 Jan. 2011

Firstpage

1059

Lastpage

1062

Abstract

We present a microfluidic dispenser chip for printing of single-particles or cells onto a substrate. The dispenser chip, fabricated in silicon/glass technology features hydrodynamic flow focusing to transport single particles in a fluid channel and a drop-on-demand dispensing mechanism to eject single particles confined in a free flying droplet of about 200 pl volume. A computer vision-based approach is employed to detect single particles prior to dispensing. We have evaluated the system experimentally by successfully depositing single polystyrene-beads on glass slides with a yield of 92%.

Keywords

bioMEMS; biological fluid dynamics; cellular biophysics; computer vision; lab-on-a-chip; microfluidics; computer vision-based approach; drop-on-demand dispensing; glass slides; hydrodynamic flow focusing; microfluidic dispenser chip; printing; silicon/glass technology; single cells; single particle transport; single particles; single polystyrene beads; substrate; Focusing; Glass; Printing; Reservoirs; Silicon; Substrates; Suspensions;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2011 IEEE 24th International Conference on

Conference_Location

Cancun

ISSN

1084-6999

Print_ISBN

978-1-4244-9632-7

Type

conf

DOI

10.1109/MEMSYS.2011.5734611

Filename

5734611