Title :
Towards a microfluidic dispenser chip for printing of single cells
Author :
Yusof, A. ; Zengerle, R. ; Koltay, P.
Author_Institution :
IMTEK, Univ. of Freiburg, Freiburg, Germany
Abstract :
We present a microfluidic dispenser chip for printing of single-particles or cells onto a substrate. The dispenser chip, fabricated in silicon/glass technology features hydrodynamic flow focusing to transport single particles in a fluid channel and a drop-on-demand dispensing mechanism to eject single particles confined in a free flying droplet of about 200 pl volume. A computer vision-based approach is employed to detect single particles prior to dispensing. We have evaluated the system experimentally by successfully depositing single polystyrene-beads on glass slides with a yield of 92%.
Keywords :
bioMEMS; biological fluid dynamics; cellular biophysics; computer vision; lab-on-a-chip; microfluidics; computer vision-based approach; drop-on-demand dispensing; glass slides; hydrodynamic flow focusing; microfluidic dispenser chip; printing; silicon/glass technology; single cells; single particle transport; single particles; single polystyrene beads; substrate; Focusing; Glass; Printing; Reservoirs; Silicon; Substrates; Suspensions;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2011 IEEE 24th International Conference on
Conference_Location :
Cancun
Print_ISBN :
978-1-4244-9632-7
DOI :
10.1109/MEMSYS.2011.5734611
