Title :
Electrodeformation for single cell mechanical characterization
Author :
Chen, Jian ; Abdelgawad, Mohamed ; Yu, Liming ; Shakiba, Nika ; Chien, Wei-Yin ; Lu, Zhe ; Geddie, William B. ; Jewett, Michael A S ; Sun, Yu
Author_Institution :
Inst. of Biomater. & Biomed. Eng., Univ. of Toronto, Toronto, ON, Canada
Abstract :
This paper presents the use of electrodeformation as a method for single cell mechanical characterization. Cells were placed between two microelectrodes with a rectangular AC electric field applied and cell deformation recorded. Numerical simulations were performed to model cell electrodeformation based on the Maxwell stress tensor formulation in which effects of cell electrical property variations on their electrodeformed behaviors were investigated. By comparing the measured morphological changes with those obtained from numerical simulations, we were able to quantify Young´s modulus of SiHa cells (601±183 Pa) and ME180 cells (1463±649 Pa), which were then verified using conventional micropipette aspiration (SiHa: 400±290 Pa and ME180: 1070±580 Pa).
Keywords :
Young´s modulus; bioelectric phenomena; biological effects of fields; biomechanics; biomedical electrodes; cellular biophysics; deformation; microelectrodes; ME180 cells; Maxwell stress tensor formulation; SiHa cells; Young´s modulus; cell deformation; cell electrical property variation; electrodeformation; microelectrodes; micropipette aspiration; morphological changes; numerical simulation; rectangular AC electric field; single cell mechanical characterization; Biomembranes; Conductivity; Electric fields; Electrodynamics; Force; Young´s modulus;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2011 IEEE 24th International Conference on
Conference_Location :
Cancun
Print_ISBN :
978-1-4244-9632-7
DOI :
10.1109/MEMSYS.2011.5734626
