Title :
An evanescent fluorescence immunosensor based on K+ and Na+-exchanged patterned optical waveguides in glass
Author :
Zhou, Y. ; Magill, J.V. ; Cushley, W. ; De La Rue, R.M. ; Laybourn, P.J.R.
Author_Institution :
Dept. of Electron. & Electr. Eng., Glasgow Univ., UK
Abstract :
The authors describe, the successful fabrication of K+ and Na+-exchanged patterned waveguides and their application to wash-free immunoassays using evanescent excitation of fluorescence. Experimental results show that the specific binding of a fluorescently labelled antibody to its appropriate antigen could be resolved at the concentration level of 1 μg/ml
Keywords :
biological techniques and instruments; biosensors; fluorescence; ion exchange; optical glass; optical waveguides; optical workshop techniques; spectrochemical analysis; K+ ion exchange; Na+ ion exchange; antigen; evanescent fluorescence immunosensor; fabrication; fluorescently labelled antibody; glass waveguides; ion exchanged waveguides; patterned optical waveguides; specific binding; wash-free immunoassays;
Conference_Titel :
Optical Techniques and Biomedical Applications, IEE Colloquium on
Conference_Location :
London
