Title :
A nanosecond pulse generator for intracellular electromanipulation
Author :
Deng, Jingdong ; Stark, Robert H. ; Schoenbach, Karl H.
Author_Institution :
Phys. Electron. Res. Inst., Old Dominion Univ., Norfolk, VA, USA
Abstract :
Intracellular electromanipulation (ICEM), the manipulation of substructures of biological cells by means of externally applied electric fields requires electrical pulses of nanosecond to tens of nanosecond duration and amplitudes of tens of kilovolt. The load in these bioelectric experiments, a cuvette containing biological cells, immersed in a solution of high conductivity, is generally on the order of 10 /spl Omega/. Strip line pulsers satisfy the condition of low impedance. A Blumlein strip line pulser with a pressurized spark gap as switch has been designed. The electrical characteristics of a particular pulse generator, which produces an 8 ns pulse with voltages between 2 kV and 30 kV across a 10 /spl Omega/ load are described.
Keywords :
bioelectric phenomena; cell motility; electric field effects; load (electric); pulse generators; pulsed power switches; pulsed power technology; spark gaps; strip lines; 10 ohm; 2 to 30 kV; 8 ns; Blumlein strip line pulser; biological cells; electrical characteristics; intracellular electromanipulation; nanosecond pulse generator; pressurized spark gap; substructure manipulation; Bioelectric phenomena; Biological cells; Conductivity; Electric variables; Impedance; Nanobioscience; Pulse generation; Sparks; Strips; Switches;
Conference_Titel :
Power Modulator Symposium, 2000. Conference Record of the 2000 Twenty-Fourth International
Conference_Location :
Norfolk, VA, USA
Print_ISBN :
0-7803-5826-0
DOI :
10.1109/MODSYM.2000.896161
