A nanosecond pulse generator for intracellular electromanipulation

Author

Deng, Jingdong ; Stark, Robert H. ; Schoenbach, Karl H.

Author_Institution

Phys. Electron. Res. Inst., Old Dominion Univ., Norfolk, VA, USA

fYear

2000

fDate

26-29 June 2000

Firstpage

47

Lastpage

50

Abstract

Intracellular electromanipulation (ICEM), the manipulation of substructures of biological cells by means of externally applied electric fields requires electrical pulses of nanosecond to tens of nanosecond duration and amplitudes of tens of kilovolt. The load in these bioelectric experiments, a cuvette containing biological cells, immersed in a solution of high conductivity, is generally on the order of 10 /spl Omega/. Strip line pulsers satisfy the condition of low impedance. A Blumlein strip line pulser with a pressurized spark gap as switch has been designed. The electrical characteristics of a particular pulse generator, which produces an 8 ns pulse with voltages between 2 kV and 30 kV across a 10 /spl Omega/ load are described.

Keywords

bioelectric phenomena; cell motility; electric field effects; load (electric); pulse generators; pulsed power switches; pulsed power technology; spark gaps; strip lines; 10 ohm; 2 to 30 kV; 8 ns; Blumlein strip line pulser; biological cells; electrical characteristics; intracellular electromanipulation; nanosecond pulse generator; pressurized spark gap; substructure manipulation; Bioelectric phenomena; Biological cells; Conductivity; Electric variables; Impedance; Nanobioscience; Pulse generation; Sparks; Strips; Switches;

fLanguage

English

Publisher

ieee

Conference_Titel

Power Modulator Symposium, 2000. Conference Record of the 2000 Twenty-Fourth International

Conference_Location

Norfolk, VA, USA

ISSN

1076-8467

Print_ISBN

0-7803-5826-0

Type

conf

DOI

10.1109/MODSYM.2000.896161

Filename

896161