A 96-well SPRI reactor in a photo-activated polycarbonate (PPC) microfluidic chip

Author

Park, Daniel S. ; Hupert, Mateusz L. ; Witek, Makgorzata ; Guy, Jason ; Datta, Proyag ; You, Byoung H. ; Soper, Steven A. ; Murphy, Michael C.

Author_Institution

Louisiana State Univ., University

fYear

2007

fDate

21-25 Jan. 2007

Firstpage

433

Lastpage

436

Abstract

A 96-well solid-phase reversible immobilization (SPRI) reactor in a micro molded, photo-activated polycarbonate (PPC) chip was designed, fabricated, and used to purify genomic DNA (gDNA) in a highly parallelized fashion. A SPRI reactor was designed to fit in a 96-well microtiter plate format with compatibility to the existing samples/reagents loading technologies. A high quality, metallic, large area mold insert (LAMI) was fabricated using a SU-8 based UV-LIGA technique. Micro molding of polycarbonate (PC) was done using hot embossing, resulting in good replication fidelity over the large surface area. The 96-well PPC-SPRI reactor, assembled by thermal fusion bonding, showed 100% efficiency for simple and fast purification of gDNA from whole cell lysates of bacterial species. The 96- well PPC-SPRI reactor will open up new avenues for low cost, disposable DNA sample purification in high throughput applications.

Keywords

DNA; biomedical equipment; embossing; genetics; microfluidics; moulding; DNA sample purification; SU-8; UV-LIGA technique; genomic DNA purification; hot embossing; large area mold insert; micro molded chip; microfluidic chip; microtiter plate; photo-activated polycarbonate; solid-phase reversible immobilization reactor; Assembly; Bioinformatics; Bonding; DNA; Embossing; Genomics; Inductors; Microfluidics; Microorganisms; Purification;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems, 2007. MEMS. IEEE 20th International Conference on

Conference_Location

Hyogo

ISSN

1084-6999

Print_ISBN

978-1-4244-095-5

Electronic_ISBN

1084-6999

Type

conf

DOI

10.1109/MEMSYS.2007.4432983

Filename

4432983