Microbiosensor for electrical impedance spectroscopic study of melanoma cells

Author

Avram, Andrei ; Marculescu, Catalin ; Balan, Catalin Mihai ; Voitincu, C. ; Pirvulescu, C. ; Volmer, M. ; Popescu, Adrian ; Mihailescu, M. ; Avram, Marioara

Author_Institution

Nat. Inst. for R&D in Microtechnologies, Bucharest, Romania

Volume

1

fYear

2012

fDate

15-17 Oct. 2012

Firstpage

165

Lastpage

168

Abstract

This paper presents the fabrication and characterization of an interdigitated microelectrode array (IDMEA) biosensor. Electrochemical impedance spectroscopy and cyclic voltammetry were used to study the cellular activities of B16 melanoma cell line C57BL, including the kinetics of cell adhesion and spreading on IDMEA. Impedance spectra of B16 melanoma cells on microelectrodes were obtained in cell 0.1 M PBS with 50 mM [Fe(CN)₍₆₎]^(3-/4-) as redox species. Randles equivalent circuits were used to model the electrochemical processes. Impedance spectra allowed us to analyze the changes in the double layer capacitance and charge transfer resistance due to cell attachment on the interdigitated microelectrodes.

Keywords

adhesion; bioelectric phenomena; biomechanics; biomedical electrodes; biomedical electronics; biosensors; cancer; capacitance; cellular biophysics; charge exchange; electrochemical impedance spectroscopy; equivalent circuits; microelectrodes; microsensors; oxidation; reaction kinetics; reduction (chemical); voltammetry (chemical analysis); wetting; B16 melanoma cell line C57BL; cell adhesion kinetics; cell attachment; cell spreading kinetics; cellular activity; charge transfer resistance; cyclic voltammetry; double layer capacitance; electrochemical impedance spectroscopy; electrochemical processes; interdigitated microelectrode array biosensor; randles equivalent circuits; redox species; Immune system; Impedance; Malignant tumors; Metals; Microelectrodes; Silicon; Interdigitated microelectrodes array (IDMEA); biosensor; cyclic voltammetry; electrochemical impedance spectroscopy; melanoma;

fLanguage

English

Publisher

ieee

Conference_Titel

Semiconductor Conference (CAS), 2012 International

Conference_Location

Sinaia

ISSN

1545-857X

Print_ISBN

978-1-4673-0737-6

Type

conf

DOI

10.1109/SMICND.2012.6400666

Filename

6400666