Adhesion-based cell sorter with antibody-immobilized functionalized-parylene surface

Author

Miwa, Junichi ; Suzuki, Yuji ; Kasagi, Nobuhide

Author_Institution

Univ. of Tokyo, Tokyo

fYear

2007

fDate

21-25 Jan. 2007

Firstpage

27

Lastpage

30

Abstract

We report the development of a micro cell sorter for extraction of rare cells from a limited amount of sample. The separation principle is based on specific adhesion with the aid of surface-immobilized antibodies. Antibody-immobilized channel walls are fabricated with a versatile technique using functionalized parylene. Performance of the cell sorter is characterized through cell velocity measurements, in which deceleration of positive cells up to 60% is observed. Cell separation with a mixture containing positive and negative cells is also demonstrated.

Keywords

adhesion; bioMEMS; biochemistry; biomedical equipment; biomedical measurement; cellular biophysics; microfluidics; molecular biophysics; patient treatment; polymers; velocity measurement; adhesion-based cell sorter; antibody-coated micro channel walls; antibody-immobilized channel walls; cell separation; cell velocity measurements; functionalized-parylene surface; micro cell sorter; microfabrication; positive cell deceleration; positive-negative cell mixtures; rare cell extraction; stem cell therapy; surface-immobilized antibodies; Cells (biology); Etching; Fluorescence; Glass; Magnetic separation; Molecular biophysics; Plugs; Sorting; Stem cells; Surface treatment;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems, 2007. MEMS. IEEE 20th International Conference on

Conference_Location

Hyogo

ISSN

1084-6999

Print_ISBN

978-1-4244-095-5

Electronic_ISBN

1084-6999

Type

conf

DOI

10.1109/MEMSYS.2007.4433050

Filename

4433050