Title :
Effect of biological cell size and shape on killing efficiency of pulsed electric field
Author :
El-Hag, Ayman ; Jayaram, Shesha H.
Author_Institution :
American Univ. of Sharjah, Sharjah
fDate :
June 30 2008-July 3 2008
Abstract :
In this study the effect of microorganism size and shape on the killing efficiency of pulsed electric field is investigated both experimentally and using a transient finite element program. The effect of cell size, membrane thickness, cell shape (spherical, elliptical and cylindrical) on the trans-membrane voltage is studied. It has been found that both the cell size and cell membrane thickness have significant effect on the induced field across the cell membrane. The findings of the simulation results have been evaluated by comparing the trends with some experimental results. Four different types of microorganisms that have different shapes and dimensions have been inoculated with water at a conductivity level of 100 muS/cm and have been treated with the application of a pulsed electric field of 70 kV/cm. Significant difference in bacteria reduction was noticed between the treated cells which could be attributed to the cell size and shape.
Keywords :
biological effects of fields; cellular biophysics; electric field effects; microorganisms; bacteria reduction; biological cell size; cell shape; microorganism size; pulsed electric field killing efficiency; transmembrane voltage; Biological cells; Biomembranes; Cells (biology); Conductivity; Equations; Finite element methods; Microorganisms; Pulse shaping methods; Shape; Voltage;
Conference_Titel :
Dielectric Liquids, 2008. ICDL 2008. IEEE International Conference on
Conference_Location :
Futuroscope-Chasseneuil
Print_ISBN :
978-1-4244-1585-4
Electronic_ISBN :
978-1-4244-1586-1
DOI :
10.1109/ICDL.2008.4622479
