Title :
3D reconstruction from electron microscope images with TxBR
Author :
Lawrence, Albert F. ; Phan, Sébastien ; Moussavi, Farshid
Author_Institution :
Univ. of California, LaJolla, CA, USA
fDate :
June 28 2009-July 1 2009
Abstract :
Tomographic reconstruction from large format electron microscope requires special procedures to handle geometric distortions arising from electron optics as opposed to light-ray optics. In particular, electrons travel in curvilinear paths through the sample and defocus and other aberrations can be more severe. We report on a software package, TxBR, which solves the inverse problem associated with a generalized ray transform. We also review the prospects for automated alignment, and discuss possible approaches.
Keywords :
Radon transforms; electron microscopy; electron optics; image reconstruction; inverse problems; medical image processing; tomography; TxBR software package; automated tracking; electron microscope 3D image reconstruction; electron optics; electron tomographic reconstruction; generalized Radon transform; generalized ray transform; geometric distortion; inverse problem; large format electron microscope; Detectors; Electron microscopy; Electron optics; Geometrical optics; Image reconstruction; Optical distortion; Optical filters; Optical microscopy; Sensor arrays; Tomography; Automated Tracking and Alignment; Bundle Adjustment; Electron Tomography; Generalized Radon Transform;
Conference_Titel :
Biomedical Imaging: From Nano to Macro, 2009. ISBI '09. IEEE International Symposium on
Conference_Location :
Boston, MA
Print_ISBN :
978-1-4244-3931-7
Electronic_ISBN :
1945-7928
DOI :
10.1109/ISBI.2009.5193256