3D reconstruction from electron microscope images with TxBR

Author

Lawrence, Albert F. ; Phan, Sébastien ; Moussavi, Farshid

Author_Institution

Univ. of California, LaJolla, CA, USA

fYear

2009

fDate

June 28 2009-July 1 2009

Firstpage

1131

Lastpage

1134

Abstract

Tomographic reconstruction from large format electron microscope requires special procedures to handle geometric distortions arising from electron optics as opposed to light-ray optics. In particular, electrons travel in curvilinear paths through the sample and defocus and other aberrations can be more severe. We report on a software package, TxBR, which solves the inverse problem associated with a generalized ray transform. We also review the prospects for automated alignment, and discuss possible approaches.

Keywords

Radon transforms; electron microscopy; electron optics; image reconstruction; inverse problems; medical image processing; tomography; TxBR software package; automated tracking; electron microscope 3D image reconstruction; electron optics; electron tomographic reconstruction; generalized Radon transform; generalized ray transform; geometric distortion; inverse problem; large format electron microscope; Detectors; Electron microscopy; Electron optics; Geometrical optics; Image reconstruction; Optical distortion; Optical filters; Optical microscopy; Sensor arrays; Tomography; Automated Tracking and Alignment; Bundle Adjustment; Electron Tomography; Generalized Radon Transform;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Imaging: From Nano to Macro, 2009. ISBI '09. IEEE International Symposium on

Conference_Location

Boston, MA

ISSN

1945-7928

Print_ISBN

978-1-4244-3931-7

Electronic_ISBN

1945-7928

Type

conf

DOI

10.1109/ISBI.2009.5193256

Filename

5193256