Title :
Automated actin filament segmentation, tracking and tip elongation measurements based on open active contour models
Author :
Li, Hongsheng ; Shen, Tian ; Smith, Matthew B. ; Fujiwara, Ikuko ; Vavylonis, Dimitrios ; Huang, Xiaolei
Author_Institution :
Dept. of Comput. Sci. & Eng., Lehigh Univ., Bethlehem, PA, USA
fDate :
June 28 2009-July 1 2009
Abstract :
This paper presents an automated method for actin filament segmentation and tracking for measuring tip elongation rates in total internal reflection fluorescence microscopy (TIRFM) images. The main contributions of the paper are: (i) we use a novel open active contour model for filament segmentation and tracking, which is fast and robust against noise; and (ii) different strategies are proposed to solve the filament intersection problem, which is shown to be the main difficulty in filament tracking. Application to experimental results demonstrated the robustness and effectiveness of this method.
Keywords :
fluorescence; image registration; image segmentation; image sequences; medical image processing; molecular biophysics; polymerisation; proteins; actin filament segmentation; actin filament tracking; open active contour model; tip elongation rates; total internal reflection fluorescence microscopy; Active contours; Fluorescence; Image segmentation; In vitro; Kinetic theory; Microscopy; Noise robustness; Polymers; Proteins; Reflection; Actin Filament; Active Contour Models; Elongation Measurement; Tracking;
Conference_Titel :
Biomedical Imaging: From Nano to Macro, 2009. ISBI '09. IEEE International Symposium on
Conference_Location :
Boston, MA
Print_ISBN :
978-1-4244-3931-7
Electronic_ISBN :
1945-7928
DOI :
10.1109/ISBI.2009.5193303
