Title :
A surface acoustic wave sensor for the study of membrane-protein/ligand interactions using whole cells
Author :
Saitakis, M. ; Dellaporta, A. ; Gizeli, Electra
Author_Institution :
Dept. of Biol., Crete Univ., Heraklion
Abstract :
Acoustic biosensors are sensitive not only to mass coupling but also to visco-elastic changes occurring close to the sensor surface. In this work, a surface acoustic wave (SAW) device, operating at 110 MHz and with a penetration depth of ~100 nm, has been applied to monitor the binding between membrane-bound proteins (HLA-A2) and surface-immobilized antibodies (anti-HLA-A2). The sensitivity of amplitude change correlated to the number of HLA-A2/antibody complexes formed on the device surface. This allowed for the calculation of the two-dimensional (2D) kinetics and affinity constant of the HLA-A2/antibody interaction based on real-time acoustic data.
Keywords :
biochemistry; biomembranes; biosensors; cellular biophysics; molecular biophysics; proteins; reaction kinetics; surface acoustic wave sensors; SAW device; acoustic biosensors; affinity constant; frequency 110 MHz; mass coupling; membrane protein-ligand interactions; protein binding; real-time acoustic data; surface acoustic wave sensor; surface-immobilized antibodies; two-dimensional kinetics; visco-elastic changes; Acoustic devices; Acoustic sensors; Acoustic waves; Biosensors; Cells (biology); Gold; Monitoring; Proteins; Surface acoustic wave devices; Surface acoustic waves;
Conference_Titel :
Frequency Control Symposium, 2008 IEEE International
Conference_Location :
Honolulu, HI
Print_ISBN :
978-1-4244-1794-0
Electronic_ISBN :
1075-6787
DOI :
10.1109/FREQ.2008.4623019
