Dielectrophoresis of plant protoplasts

Author

Kurinobu, Shuntaro

Author_Institution

Dept. of Electr. Eng., Fukuyama Univ., Japan

fYear

1991

fDate

Sept. 28 1991-Oct. 4 1991

Firstpage

660

Abstract

The spectra of effective dielectric constants which are proportional to dielectrophoretic force were investigated for a single protoplast of Ducaus carota in the range of 100 kHz to 50 MHz. The dielectrophoretic force was obtained by measuring the velocity of protoplast using an electrode which provides for a constancy in the dielectrophoretic force. The spectra show two or three peaks. In addition, the spectra washed in sucrose solution are found to be different from those washed and suspended in mannitol solution. the spectrum of the effective dielectric constants is obtained by measuring the impedance of a suspension in the range of 100 kHz to 10 MHz. The spectrum obtained is compared with that of the velocity measurement method.<>

Keywords

bioelectric phenomena; cellular biophysics; electric fields; electrodes; electrophoresis; 100 kHz to 10 MHz; 100 kHz to 50 MHz; Ducaus carota; dielectric constants; dielectrophoretic force; electrode; impedance; plant protoplasts; spectra; Cities and towns; Dielectric constant; Dielectric measurements; Dielectrophoresis; Drag; Electrodes; Force measurement; Impedance measurement; Sugar; Velocity measurement;

fLanguage

English

Publisher

ieee

Conference_Titel

Industry Applications Society Annual Meeting, 1991., Conference Record of the 1991 IEEE

Conference_Location

Dearborn, MI, USA

Print_ISBN

0-7803-0453-5

Type

conf

DOI

10.1109/IAS.1991.178249

Filename

178249