Title :
Optical copper(II) sensor based on immobilised salicylic acid in sol-gel film and fluorescence quenching measurement
Author :
Sundari, Rita ; Ahmad, Musa ; Heng, Lee Yook
Author_Institution :
Sch. of Chem. Sci. & Food Technol, Univ. Kebangsaan Malaysia, Selangor, Malaysia
Abstract :
An optical copper (II) sensor has been developed in this study by applying immobilized salicylic acid in sol gel film as reagent phase, based on fluorescence quenching measurement. It has been known that copper displays important role in enzymatic metabolism in the human body. Promising results were achieved under optimum pH 5.0, when the fluorescent signal was measured at excitation and emission wavelengths of 350 nm and 440 nm, respectively. The linearity was found in the Cu(II) concentration range of 5.0 - 80.0 × 10-6 M with LOD of 4.4 × 10-6 M. Nine repetitive measurements yielded good repeatability (3.49 % RSD) and reproducibility (7.25 % RSD). Intramolecular photoautomerism of salicylic acid related to thermodynamic reaction observed in this study showed another important feature in chemical sensing.
Keywords :
biomedical measurement; chemical sensors; copper; enzymes; molecular biophysics; optical sensors; pH; patient diagnosis; radiation quenching; sol-gel processing; 350 to 440 nm; Cu; enzymatic metabolism; fluorescence quenching; immobilised salicylic acid; intramolecular photoautomerism; optical copper sensor; sol-gel film; thermodynamic reaction; Biochemistry; Copper; Displays; Fluorescence; Humans; Linearity; Optical films; Optical sensors; Phase measurement; Wavelength measurement; copper (II) sensing; fluorescence quenching; salicylic acid; sol gel film;
Conference_Titel :
Sensors and the International Conference on new Techniques in Pharmaceutical and Biomedical Research, 2005 Asian Conference on
Print_ISBN :
0-7803-9370-8
DOI :
10.1109/ASENSE.2005.1564504
