Title :
Screening of single Escherichia coli by electric field and laser tweezer
Author :
Morishima, Keisuke ; Arai, Fumihito ; Fukuda, Toshio ; Yoshikawa, Kenichi
Author_Institution :
Dept. of Micro Syst. Eng., Nagoya Univ., Japan
Abstract :
In this paper, we propose a novel methodology for high throughput screening of microbes. We have developed a prototype of a microchannel system for high throughput screening of Escherichia coli. Experimental demonstrations of noncontact transportation and manipulation of Escherichia coli by dielectrophoretic force and radiation pressure of laser tweezers were carried out with a laser manipulator system. We discuss research issues in bio-micromanipulation and present a new direction in this field. We consider the basic strategies to improve the working efficiency and the operability of bio-micromanipulation. In experiments, we show that transportation and separation of Escherichia coli with electric field and optical radiation pressure is profitable for future practical application in high throughput screening of microbes
Keywords :
biological techniques; biotechnology; electrophoresis; microfluidics; micromanipulators; microorganisms; radiation pressure; separation; bio-micromanipulation; dielectrophoretic force; high throughput screening; laser manipulator system; laser tweezers; microbes; microchannel system; noncontact transportation; operability; radiation pressure; separation; single Escherichia coli; Biomedical engineering; Biomedical optical imaging; DNA; Dielectrophoresis; High speed optical techniques; Humans; Microchannel; Microorganisms; Robots; Throughput;
Conference_Titel :
Micromechatronics and Human Science, 1997. Proceedings of the 1997 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
0-7803-4171-6
DOI :
10.1109/MHS.1997.768874
