High-throughput micro capillary electrophoresis chip for bio-analytical application utilizing multi-wavelength detection

This paper presents a novel microfluidic device for high-throughput capillary electrophoresis (CE) analysis utilizing multi-wavelength detection. In general, fluorescent dye can only be excited using a specific range of wavelength and then it will emit specific fluorescence signal with a longer wavelength. In this study, pairs of multi-mode optic fiber are embedded at the downstream of the separation channel of the CE chips for multiple-wavelength fluorescence detection. The proposed device could detect multiple samples with different kinds of fluorescence labeling in the same channel in a single run. Experimental results show that a mixture of Rhodamine B and FITC fluorescein can be detected individually while using different kinds of light source for fluorescence excitation. Sample moving speed in the microchannel and DNA fragments are also measured and detected using the proposed device.