A low voltage single cell electroporator with a microfabricated sense-porate aperture

Author

Sarkar, Anirban ; Mitra, Bivas ; Shastry, Ashutosh ; Wadia, Sohrab ; Mulherkar, R. ; Lal, Rakesh

Author_Institution

Dept. of Electr. Eng., Indian Inst. of Technol., Bombay, India

fYear

2004

fDate

2004

Firstpage

375

Lastpage

378

Abstract

We present a novel flow-type single cell electroporation (SCE) system for low voltage electroporation of biological cells. We have used a microfabricated silicon sense-porate aperture to detect and identify a cell by its impedance and then apply the optimum electric field on it. Incorporation of a fluorescent dye into mouse embryo fibroblast NIH-3T3 cells has been demonstrated with a cell survival rate or viability much higher than conventional macro-electroporators. This paper reports the principle, design and implementation of this system and the experimental results obtained using it.

Keywords

biological techniques; biosensors; cellular biophysics; elemental semiconductors; micromachining; silicon; NIH-3T3 cells; Si; biological cells; cell survival rate; flow type single cell electroporation; fluorescent dye; macro electroporators; microfabricated silicon sense porate aperture; mouse embryo fibroblast; Apertures; Biological cells; Cells (biology); Embryo; Fibroblasts; Fluorescence; Impedance; Low voltage; Mice; Silicon;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems, 2004. 17th IEEE International Conference on. (MEMS)

Print_ISBN

0-7803-8265-X

Type

conf

DOI

10.1109/MEMS.2004.1290600

Filename

1290600