An investigation of thermally induced collagen structural transitions by second harmonic generation microscopy

Author

Sun, Yen ; Sun-Jan Lin ; Hsiao, Chih-Yuan ; Lo, Wen ; Jee, Shiou-Hwa ; Dong, Chen-Yuan

Author_Institution

Dept. of Phys., Nat. Taiwan Univ., Taipei, Taiwan

fYear

2004

fDate

14-17 Dec. 2004

Firstpage

177

Lastpage

178

Abstract

In this work, the thermal disruption of type I collagen is investigated using second harmonic generation (SHG) microscopy. We investigated the effects of second harmonic generation images and intensity of rat tail tendon in the temperature range from 25 to 60°C. We found a structural transition at 57°C where a drop in second harmonic intensity is correlated to a disorganization of the collagen fibers. Our results suggest that SHG microscopy is an effective technique to monitor thermally induced collagen structural changes for biomedical applications.

Keywords

biomedical optical imaging; biothermics; macromolecules; molecular biophysics; molecular configurations; multiphoton spectroscopy; optical harmonic generation; optical microscopy; proteins; 25 to 60 C; SHG microscopy; biomedical applications; collagen fiber disorganization; rat tail tendon; second harmonic generation microscopy; thermal disruption; thermally induced collagen structural transitions; type I collagen; Biomedical imaging; Fluorescence; Frequency conversion; Laser stability; Optical harmonic generation; Optical imaging; Optical microscopy; Tail; Temperature distribution; Tendons;

fLanguage

English

Publisher

ieee

Conference_Titel

Biophotonics, 2004. APBP 2004. The Second Asian and Pacific Rim Symposium on

Print_ISBN

0-7803-8676-0

Type

conf

DOI

10.1109/APBP.2004.1412339

Filename

1412339