Title :
High-resolution spectroscopic photoacoustic tomography for noninvasive functional imaging of small-animal brains in vivo
Author :
Wang, Lihong V. ; Wang, Xueding ; Ku, Geng ; Xie, Xueyi ; Stoica, George
Author_Institution :
Dept. of Biomed. Eng., Texas A&M Univ., College Station, TX, USA
Abstract :
Based on the multiwavelength laser-based photoacoustic tomography, noninvasive imaging of cerebral blood oxygenation and blood volume in small-animal brains in vivo was realized. The high sensitivity of this technique is based on the spectroscopic differences between oxy- and deoxy-hemoglobins whereas its spatial resolution is diffraction-limited by the photoacoustic signals. The point-by-point distributions of hemoglobin oxygen saturation and total concentration of hemoglobin in the cerebral cortical venous vessels, altered by systemic physiological modulations including hyperoxia and hypoxia, were visualized successfully through the intact skin and skull. This technique can potentially accelerate the progress in neuroscience and provide important new insights into cerebrovascular physiology and brain function.
Keywords :
acoustic tomography; biochemistry; biomedical optical imaging; biomedical ultrasonics; blood vessels; brain; laser applications in medicine; molecular biophysics; neurophysiology; optical tomography; proteins; blood volume; brain function; cerebral blood oxygenation; cerebral cortical venous vessel; cerebrovascular physiology; deoxy-hemoglobin; hemoglobin oxygen saturation; hyperoxia; hypoxia; multiwavelength laser; neuroscience; noninvasive functional imaging; physiological modulation; point-by-point distribution; small-animal brain; spatial resolution; spectroscopic photoacoustic tomography; Blood; Diffraction; High-resolution imaging; In vivo; Skin; Skull; Spatial resolution; Spectroscopy; Tomography; Visualization;
Conference_Titel :
Biophotonics, 2004. APBP 2004. The Second Asian and Pacific Rim Symposium on
Print_ISBN :
0-7803-8676-0
DOI :
10.1109/APBP.2004.1412374