The trial of number measurement of the pollen Cryptomeria japonica using photoacoustic microscope

The Cryptomeria japonica(CJ) is one of the most famous evergreen needle-leaf trees in far-east Asia, especially in China, Korea and Japan, because it is known to induce allergic reaction function in the eyes and nose like hogweeds. At the previous symposium, a photoacoustic (PA) microscope was applied to measure the amount of the CJ pollen. The correlation between PA signals versus CJ pollen amount showed the value of 0.94, and resulted the calibration curve. In this paper, the pollen was collected and fixed on the albumen (egg white) on a slide-glass set in a PA cell to reduce the background so that even a single particle of the CJ pollen particles could be measured. The obtained correlation coefficient was better than 0.97.