Title :
An MCP-based system for beta autoradiography
Author :
Lees, J.E. ; Pearson, J.F. ; Fraser, G.W. ; Hales, J.M. ; Richards, P.G.
Author_Institution :
Dept. of Phys. & Astron., Leicester Univ., UK
Abstract :
Describes a pre-commercial prototype autoradiography system which can be used to image a variety of radiolabelled biological samples, ranging from lymphocyte proliferation assays to semi-thin whole body tissue slices. These samples can be imaged with high spatial resolution (~70 microns) and high sensitivity (e.g. 3H-minimum detectable activity 0.001 Bq in 20 hours) over a wide (5-6 orders of magnitude) dynamic range. Specimens are automatically presented, through a vacuum load-lock, to an imaging microchannel plate (MCP) detector operating in high (~10-6 mbar) vacuum. The authors present images obtained from tritium labelled samples and discuss the importance of the new system for a variety of biological techniques
Keywords :
biological techniques; biological tissues; blood; radioisotope imaging; 0.001 becquerel; 20 h; 70 mum; MCP-based system; automatically presented specimens; beta autoradiography; dynamic range; high vacuum; imaging microchannel plate detector; lymphocyte proliferation assays; minimum detectable activity; pre-commercial prototype autoradiography system; radiolabelled biological samples; semi-thin whole body tissue slices; spatial resolution; tritium labelled samples; vacuum load-lock; Cameras; Detectors; Dynamic range; Electrokinetics; High-resolution imaging; Microchannel; Pixel; Pollution measurement; Prototypes; Spatial resolution;
Conference_Titel :
Nuclear Science Symposium, 1998. Conference Record. 1998 IEEE
Conference_Location :
Toronto, Ont.
Print_ISBN :
0-7803-5021-9
DOI :
10.1109/NSSMIC.1998.773831
