Title :
Evaluation of autofocus functions of conventional sputum smear microscopy for tuberculosis
Author :
Kimura, Almir ; Costa, Marly G F ; Filho, Cicero F F Costa ; Fujimoto, Luciana B M ; Salem, Julia
Author_Institution :
Centro de P&D em Tecnol. Eletron. e da Informacao, Univ. Fed. do Amazonas, Manaus, Brazil
fDate :
Aug. 31 2010-Sept. 4 2010
Abstract :
This article presents a systematic analysis of focus functions in conventional sputum smear microscopy for tuberculosis. This is the first step in the development of automatic microscopy. Nine autofocus functions are analyzed in a set of 1200 images with varying degrees of content density. These functions were evaluated using quantitative procedures. The main accomplishment of this work was to show that an autofocus function based on variance measures produced the best results for tuberculosis images.
Keywords :
biomedical optical imaging; diseases; medical image processing; optical focusing; optical microscopy; autofocus functions; automatic microscopy; conventional sputum smear microscopy; tuberculosis images; variance measures; Accuracy; Discrete wavelet transforms; Entropy; Focusing; Microscopy; Optical microscopy; Pixel; Algorithms; Equipment Design; False Positive Reactions; Humans; Image Interpretation, Computer-Assisted; Image Processing, Computer-Assisted; Microscopy; Models, Statistical; Reproducibility of Results; Robotics; Sputum; Tuberculosis;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2010 Annual International Conference of the IEEE
Conference_Location :
Buenos Aires
Print_ISBN :
978-1-4244-4123-5
DOI :
10.1109/IEMBS.2010.5626143
