Anomalous diffusion of kv2.1 channels observed by single molecule tracking in live cells

Author

Weigel, Aubrey V. ; Tamkun, Michael M. ; Krapf, Diego

Author_Institution

Sch. of Biomed. Eng., Colorado State Univ., Fort Collins, CO, USA

fYear

2010

fDate

Aug. 31 2010-Sept. 4 2010

Firstpage

3005

Lastpage

3008

Abstract

Kv2.1 are voltage gated potassium channels that form long-lived clusters on the surface of mammalian cells. We have used single molecule tracking to study the interesting dynamics of these channels in live HEK cells. Both the channels inside the clusters and non-clustering channels are found to follow anomalous subdiffusion. The effect of actin cytoskeleton on the diffusion properties of the channels is also investigated in the presence of cytochalasin D, a F-actin binding drug that blocks actin polymerization.

Keywords

biochemistry; biodiffusion; bioelectric phenomena; biomembrane transport; drugs; kidney; polymerisation; proteins; F-actin binding drug; Kv2.1 channels; actin cytoskeleton; actin polymerization; anomalous diffusion; cytochalasin D; live HEK cells; long-lived clusters; mammalian cells; single molecule tracking; voltage gated potassium channels; Biomembranes; Cells (biology); Drugs; Fluorescence; Imaging; Quantum dots; Trajectory; Actins; Algorithms; Cell Line; Cluster Analysis; Cytochalasin D; Diffusion; Green Fluorescent Proteins; Humans; Microscopy, Fluorescence; Quantum Dots; Reproducibility of Results; Shab Potassium Channels; Time Factors; Transfection;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society (EMBC), 2010 Annual International Conference of the IEEE

Conference_Location

Buenos Aires

ISSN

1557-170X

Print_ISBN

978-1-4244-4123-5

Type

conf

DOI

10.1109/IEMBS.2010.5626150

Filename

5626150