Study on Preparation Condition Optimization and Kinetics for Biodemulsifier

Using strain JM-1 producing biodemulsifier isolated from cold rolling plant emulsion wastewater as the starting strain, the optimum preparation conditions of the biodemulsifier were obtained by the experiment of carbon source, nitrogen source, trace element, and ratio of carbon to nitrogen. The concentration of the lipopeptide reached 1.62 g/L, which was 22.73% higher than un-optimized. The biodemulsifier preparation kinetics were investigated. The kinetic models of strains growth and lipopeptide formation were established by Matlab, providing a good description of temporal variations of biomass, and product in JM-1 fermentation. These models were applied to guide the industrial production of the biodemulsifier.