Manipulation of biological objects in micromachined structures

Author

Washizu, Masao

Author_Institution

Dept. of Electr. Eng. & Electron., Seikei Univ., Japan

fYear

1992

fDate

4-7 Feb 1992

Firstpage

196

Lastpage

201

Abstract

Several methods for the manipulation of biological objects are developed using micromachined structures. The electrostatic effects under high-frequency field, such as dielectrophoresis, electrostatic orientation and electrorotation, are used for the actuation of the objects, by which (1) cell handling, separation, and their fusion, (2) stretching of DNA molecules and their fixation onto substrate, (3) manipulation of protein molecules, and (4) fabrication of membrane structures, etc. are realized. A high-intensity field, in excess of 10 kV/cm, is required for the manipulation of molecules to overcome thermal randomization, which is obtained only with microfabricated electrode gaps

Keywords

bioelectric phenomena; biological techniques and instruments; cellular biophysics; mechatronics; micromechanical devices; molecular biophysics; AC electric field; cell handling; dielectrophoresis; electrorotation; electrostatic effects; electrostatic orientation; fabrication of membrane structures; high-frequency field; high-intensity field; manipulation of biological objects; manipulation of molecules; manipulation of protein molecules; microfabricated electrode gaps; micromachined structures; stretching of DNA molecules; thermal randomization overcoming; Biomembranes; Chemicals; Electrodes; Electrostatics; Fabrication; Insulation; Optical microscopy; Polarization; Proteins; Voltage;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems, 1992, MEMS '92, Proceedings. An Investigation of Micro Structures, Sensors, Actuators, Machines and Robot. IEEE

Conference_Location

Travemunde

Print_ISBN

0-7803-0497-7

Type

conf

DOI

10.1109/MEMSYS.1992.187717

Filename

187717