Title :
Mapping of Three-Dimension Optical Force Field on Micro-particle in Optical Tweezers
Author :
Wei, Ming-Tzo ; Karmenyan, Artahses ; Chiou, Arthur
Author_Institution :
Institute of Biophotonics Engineering, National Yang-Ming University, No. 155, Sec. 2, Lih-Nong Street, Taipei, Taiwan u8914017@msg.ndhu.edu.tw
Abstract :
We use a pair of orthogonal tracking beams to track and analyze the Brownian motion of a micro-particle or a living cell trapped in optical tweezers to map the three-dimensional optical force field on these samples. For optical trapping with λ = 532nm, NA = 0.85, and total power = 100mW, the axial and the transverse spring constants are around 1x10-8N/m and 2x10-8N/m, respectively for a silica particle (diameter = 2.58 μm), and 3x10-8N/m and 2x10-7N/m, respectively for a Chinese hamster ovary cell (diameter ∼15 μm).
Keywords :
Chinese hamster ovary (CHO) cell; Optical tweezers; Orthogonal tracking; three-dimension optical force field; Biomedical optical imaging; Cells (biology); Charge carrier processes; Laser beams; Lenses; Optical noise; Optical scattering; Particle beams; Particle tracking; Silicon compounds; Chinese hamster ovary (CHO) cell; Optical tweezers; Orthogonal tracking; three-dimension optical force field;
Conference_Titel :
Lasers and Electro-Optics, 2005. CLEO/Pacific Rim 2005. Pacific Rim Conference on
Print_ISBN :
0-7803-9242-6
DOI :
10.1109/CLEOPR.2005.1569668
