Laser Manipulation and Fabrication of Functional Microtool Using Photo-crosslinkable Resin

In recent years, single cell experiment has become very important for investigating unknown cell properties. We developed a novel technique to investigate individual cell properties on a chip using newly developed cell manipulation method by laser tweezers with the photo - crosslinkable resin. We propose new fabrication method of functional microtools, such as colored microtool, fluorescent microtool, cell binding microtool, rotation free microtool, rope shaped microtool, on a chip. These microtools are made of photo - crosslinkable resin. Colored microtool and fluorescent microtool are for cell manipulation using Image processing with low cost. Cell binding microtool is made for high - speed transportation of target cell. Rotation free microtool is made for position control and precise force measurement of cell and DNA. Rope shaped microtool is for versatile manipulation. Laser tweezers is employed to position control of microtools. We used mercury lamp for UV - ray illumination at the local area for combination of microtools. We succeeded in fabrication of our functional microtools and manipulation of cell in the microchip.