Title :
Quartz-based photonic crystal surfaces for multiplexed cancer biomarker detection
Author :
Huang, Chiun-Sheng ; Chaudhery, Vikram ; Polans, J. ; Lu, Min ; Cunningham, Brian T. ; Pokhriyal, Anusha ; George, Saly ; Zangar, R.C.
Author_Institution :
Dept. of Electr. & Comput. Eng., Univ. of Illinois at Urbana-Champaign, Urbana, IL, USA
Abstract :
We report for the first time the combination of a quartz-based photonic crystal (PC) with a detection instrument designed to perform label-free (LF) and enhanced fluorescence (EF) imaging for antibody microarray applications. Label-free detection is used to quantify binding density of immobilized capture antibodies. Angle-scanning approach is implemented to achieve more uniform fluorescence enhancement by reducing the coefficient of variation of replicate assays by 20-99% compared to ordinary fluorescence microscopy.
Keywords :
biomedical optical imaging; cancer; fluorescence spectroscopy; photonic crystals; quartz; SiO2; antibody microarray application; enhanced fluorescence imaging; label free fluorescence imaging; multiplexed cancer biomarker detection; quartz based photonic crystal surface; Arrays; Couplings; Fluorescence; Imaging; Instruments; Lighting; Photonic crystals;
Conference_Titel :
Sensors, 2012 IEEE
Conference_Location :
Taipei
Print_ISBN :
978-1-4577-1766-6
Electronic_ISBN :
1930-0395
DOI :
10.1109/ICSENS.2012.6411036
