Quartz-based photonic crystal surfaces for multiplexed cancer biomarker detection

Author

Huang, Chiun-Sheng ; Chaudhery, Vikram ; Polans, J. ; Lu, Min ; Cunningham, Brian T. ; Pokhriyal, Anusha ; George, Saly ; Zangar, R.C.

Author_Institution

Dept. of Electr. & Comput. Eng., Univ. of Illinois at Urbana-Champaign, Urbana, IL, USA

fYear

2012

fDate

28-31 Oct. 2012

Firstpage

1

Lastpage

4

Abstract

We report for the first time the combination of a quartz-based photonic crystal (PC) with a detection instrument designed to perform label-free (LF) and enhanced fluorescence (EF) imaging for antibody microarray applications. Label-free detection is used to quantify binding density of immobilized capture antibodies. Angle-scanning approach is implemented to achieve more uniform fluorescence enhancement by reducing the coefficient of variation of replicate assays by 20-99% compared to ordinary fluorescence microscopy.

Keywords

biomedical optical imaging; cancer; fluorescence spectroscopy; photonic crystals; quartz; SiO₂; antibody microarray application; enhanced fluorescence imaging; label free fluorescence imaging; multiplexed cancer biomarker detection; quartz based photonic crystal surface; Arrays; Couplings; Fluorescence; Imaging; Instruments; Lighting; Photonic crystals;

fLanguage

English

Publisher

ieee

Conference_Titel

Sensors, 2012 IEEE

Conference_Location

Taipei

ISSN

1930-0395

Print_ISBN

978-1-4577-1766-6

Electronic_ISBN

1930-0395

Type

conf

DOI

10.1109/ICSENS.2012.6411036

Filename

6411036