A new intact immobilization of liposome as sensing bio nano-particle on oxidized metal electrode surface

We newly report that the intactness of liposome on the electrode surfaces of Pt and Au metals could be significantly improved on thermally oxidized metal surfaces, compared to those with non-oxidization or even with a SAM treatment. It is especially noted that the sub-gel phase of lipid membrane of the liposome on the oxidized Pt surface is much more suppressed than the case of non-oxidized, indicating that the fluidity of intact liposome greatly improves, as the sub-gel phase then crystalline phase will increase when the intact liposome loses its closed spherical shape. Finally, the retention time of the intact liposome on the oxidized surface becomes improved to about one day. These results indicate that the oxidation treatment made the metal surface hydrophilic enough to keep the stable and intact liposome. We think that the retention time up to one day is good enough for practical biosensor applications using intact liposome to improve device performance and its reliabilities.