Title :
Role of substrate microstructure on osteogenic differentiation of mesenchymal stem cells
Author :
Jabbari, Esmaiel
Author_Institution :
Univ. of South Carolina, USA
fDate :
Aug. 31 2010-Sept. 4 2010
Abstract :
The objective of this work was to investigate the effect of substrate microstructure (planar versus microtubular geometry) on the extent of osteogenic differentiation of bone mesenchymal stem (BMS) cells. For laminated sheets, the BMS cell suspension was seeded on the sheets and cultured in complete osteogenic media. For laminated microtubes, the BMS cell suspension was injected in the tubes and allowed to incubate in osteogenic media. BMS cells cultured in osteon-mimetic microtubes had drastically higher ALPase activity and calcium content, compared to laminated sheets. The data demonstrate that the 3D environment of the microtubes profoundly affects osteogenic differentiation of the BMS cells, compared to the 2D laminated sheets.
Keywords :
biomedical materials; bone; cellular biophysics; laminates; prosthetics; substrates; 2D laminated sheets; ALPase activity; BMS cell suspension; bone; calcium content; laminated microtubes; mesenchymal stem cells; microtubular geometry; osteogenic differentiation; osteogenic media; osteon-mimetic microtubes; planar geometry; substrate microstructure; Bones; Calcium; Cells (biology); Electron tubes; Helium; Laminates; Three dimensional displays; Animals; Cell Differentiation; Cells, Cultured; Equipment Design; Equipment Failure Analysis; Male; Mesenchymal Stem Cells; Osteoblasts; Osteogenesis; Rats; Rats, Wistar; Tissue Engineering; Tissue Scaffolds;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2010 Annual International Conference of the IEEE
Conference_Location :
Buenos Aires
Print_ISBN :
978-1-4244-4123-5
DOI :
10.1109/IEMBS.2010.5627489
